- Crossref DOI Prefix: 10.31695/IJASRE
- ISSN: 2454 - 8006
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International Journal of Advances in Scientific Research and Engineering-IJASRE
Physicochemical Comparison between Glass Surface and Zinc Oxide Surface: Study of Microbial Adhesion
Article Category: Industrial Engineering
Author: Youssef NAJIH,Mustapha ADAR,Noureddine KOUIDER,Jamaa BENOURRAM ,Mustapha MABROUKI
Abstract: The general context of this work is to study the effect of physicochemical properties on bacterial adhesion. In this case, the adhesion of Staphylococcus aureus (gram-positive bacteria) has been studied on a thin layer of zinc oxide (ZnO). This layer was deposited on glass substrates by sputtering under different powers (150, 200 and 250 watts). The contact angle measurements were used to calculate the surface energy components of each substrate (dispersive and polar) and to define the hydrophilic/hydrophobic character by the calculation of the free energy of interaction. Analysis of adherent cells on the surface of each substrate was made by the atomic force microscope according to the time of suspension. The results showed that the glass surface has a large polar component; against the surface of the ZnO film has a significant dispersive component. This shows that the glass surface is governed by short-range forces (Lewis acid–bases forces) while ZnO film is governed by long-range forces (van der Waals forces). Images obtained by atomic force microscopy revealed that the ZnO layer has an antibacterial effect against S. aureus.
Keyword: Staphylococcus Aureus, Surface Energy, Atomic Force Microscopy, Zinc Oxide, Contact Angle.
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