International Journal of Advances in Scientific Research and Engineering-IJASRE

Probiotic Lactobacillus species Inhibitory Effect on The Growth of Oral Streptococci

Article Category: Biotechnology

DOI: 10.31695/IJASRE.2020.33768

Pages: 125-129

Author: Wah Wah Than,Saw Sandar Maw,Zaw Khaing Oo,Reemon Htun

Abstract: Oral and dental infections are among the most prevalent infections of man. All people suffer from dental caries at some phase of their life span. The mouth is indeed an important source of infections and poor oral health affects a variety of systemic diseases. The aim of this research is to isolate, identify acid-producing lactic acid bacteria (LAB) from fermented fruits juice and followed by the 16S rRNA gene sequences of the isolated bacterial strains Lactobacillus Plantarum S1, Leuconostoc mesenteroides (SC and PP) and Burkholderia cenocepia NP were compared with reference strain sequences. Oral Streptococci were important in the etiology of dental caries. We targeted for isolation and biochemical identification of oral bacteria (isolates grown in SMAB agar) and screened antibacterial activity (agar well diffusion assay) of Lactobacillus species against oral Streptococci. Two Lactobacillus isolates (S1 and PP) were observed to behave a good antagonistic activity against oral Streptococcus strain with differences in the size of inhibition zone (mm). The zone diameter of NP broth against Streptococcus sp. was 10 mm. Oral Streptococci was not inhibited by SC broth. In this study, Leuconostoc mesenteroides (PP) showed the highest inhibition zone (13 mm) against oral Streptococci.               

Keyword: Lactic Acid Bacteria, Antibacterial Activity, Dental Caries, Oral Streptococci, Biochemical Identification

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