@article{Ahadul Putra_Minda Azhar_Iryani_Yuni Ahda_Fernita Puspasari_Ihsanawati_Dessy Natalia_2020, title={Cloning, Sequencing and Analyzing of 16S rRNA Gene from Inulin Hydrolyzing Bacteria }, volume={6}, url={https://ijasre.net/index.php/ijasre/article/view/983}, DOI={10.31695/IJASRE.2020.33790}, abstractNote={<p><em>Gen</em><em>e</em><em> of </em><em>16S rRNA is used</em><em> for molecular identification of bacteria. </em><em>This research aim</em><em>s</em><em> to analy</em><em>ze the sequences</em><em> of 16S rRNA gene from </em><em>inulin hydrolyzing bacteria of</em><em> UKG isolate. The 16S rRNA gene was isolated by PCR using BacF1 and UniB1 primers. The gene was cloned to pGEM-T Easy vector with E.coli TOP10F</em><em> as </em><em>host cell.</em> <em>Recombinant DNA was sequenced using T7</em> <em>and SP6 primer</em><em>s</em><em>. The</em> <em>size of the nucleotide base of the recombinant DNA</em><em> of the sequencing result </em><em>using SP6 primer </em><em>was</em><em> 1167 bp, whereas using T7 primer </em><em>was</em><em> 1218 bp</em><em>. The both of</em><em> the sequence</em><em>s overlapped</em><em> 760 bp. </em><em>The size of </em><em>16S rRNA gene </em><em>in the sequences was </em><em>found</em> <em>1501 bp. Recombinant DNA</em><em> that was</em> <em>restricted</em> <em>using</em> <em>EcoR1 showed 3 bands on agarose gel</em><em>.</em> <em>T</em><em>hey </em><em>were</em><em> 3000 bp, 800 bp, and 700 bp. The position of the EcoR1 palindrome sequence on the 16S rRNA gene was </em><em>at</em><em> the</em><em> 832-837</em><em>. The 16S rRNA gene also has</em><em> been</em><em> known by HindIII, BamHI, BalI, HaeIII, and SmaI</em> <em>restriction enzyme</em><em>s</em><em>.</em></p>}, number={5}, journal={ International Journal of Advances in Scientific Research and Engineering (IJASRE), ISSN:2454-8006, DOI: 10.31695/IJASRE}, author={Ahadul Putra and Minda Azhar and Iryani and Yuni Ahda and Fernita Puspasari and Ihsanawati and Dessy Natalia}, year={2020}, month={May}, pages={88–95} }